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Image Search Results
Journal: World Journal of Gastrointestinal Oncology
Article Title: BRAF V600E mutant colorectal cancer cells mediate local immunosuppressive microenvironment through exosomal long noncoding RNAs
doi: 10.4251/wjgo.v13.i12.2129
Figure Lengend Snippet: Relationship between BRAF V600E mutation and microvascular density, microlymphatic vessel density, tumor-associated macrophages, and cancer-associated fibroblasts. A: Expression of CD31 and lymphatic vessel endothelial hyaluronic acid receptor-1 (LYVE-1) in BRAF V600E mutant and BRAF wild type colorectal cancer (CRC) tissues (CD31 labeled microvascular density, and LYVE-1 labeled microlymphatic vessel density; immunohistochemistry, × 400); B: Expression of CD68 and CD163 proteins in BRAF V600E mutant and BRAF wild type CRC tissues (CD68 labeled TAMs, and CD163 labeled M2 subtype macrophages; immunohistochemistry, × 400); C: Expression of α-SMA protein in BRAF V600E mutant and BRAF wild type CRC tissues (α-SMA labeled CAFs; immunohistochemistry, × 400).
Article Snippet: After deparaffinization and hydration, antigen retrieval, and incubation in 3% H 2 O 2 for 10 min, the corresponding antibodies were added, including rabbit anti-human CD31 monoclonal antibody (1:2000, Arigo, Hsinchu City, Taiwan), rabbit LYVE-1 polyclonal antibody (1:200, Arigo), mouse anti-human CD68 monoclonal antibody (1:100, BD Biosciences, NJ, United States), rabbit anti-human CD163 monoclonal antibody (1:100, HUABIO, Hangzhou, China), and
Techniques: Mutagenesis, Expressing, Labeling, Immunohistochemistry
Journal: World Journal of Gastrointestinal Oncology
Article Title: BRAF V600E mutant colorectal cancer cells mediate local immunosuppressive microenvironment through exosomal long noncoding RNAs
doi: 10.4251/wjgo.v13.i12.2129
Figure Lengend Snippet: Effect of exosomes derived from BRAF V600E mutant colorectal cancer cells on the phenotype and secretion function of tumor-associated macrophages and cancer-associated fibroblasts. A: Effect of silencing BRAF gene on expression of CD163 in macrophages induced by exosomes derived from BRAF V600E mutant colorectal cancer cells; B: ELISA was used to detect expression of interleukin (IL)-6 and transforming growth factor (TGF)-β1 in the supernatant of THP-1 cells; C: Western blotting was used to detect expression of FAP and α-SMA in MRC-5 cells; D: ELISA was used to detect expression of IL-6, TGF-β1, and vascular endothelial growth factor in the supernatant of MRC-5 cells. a P < 0.05. VEGF: Vascular endothelial growth factor; IL: Interleukin; TGF: Transforming growth factor.
Article Snippet: After deparaffinization and hydration, antigen retrieval, and incubation in 3% H 2 O 2 for 10 min, the corresponding antibodies were added, including rabbit anti-human CD31 monoclonal antibody (1:2000, Arigo, Hsinchu City, Taiwan), rabbit LYVE-1 polyclonal antibody (1:200, Arigo), mouse anti-human CD68 monoclonal antibody (1:100, BD Biosciences, NJ, United States), rabbit anti-human CD163 monoclonal antibody (1:100, HUABIO, Hangzhou, China), and
Techniques: Derivative Assay, Mutagenesis, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot
Journal: Gastroenterology Research and Practice
Article Title: Peritoneal Metastatic Cancer Stem Cells of Gastric Cancer with Partial Mesenchymal-Epithelial Transition and Enhanced Invasiveness in an Intraperitoneal Transplantation Model
doi: 10.1155/2020/3256538
Figure Lengend Snippet: Immunohistochemistry of E-cad, α -SMA, and Snail (×200) for the two generations of intraperitoneally transplanted tumors from the pMCSC-tGC [G1] and pMCSC-tGC [G2] .
Article Snippet: The reagents used for immunohistochemical staining included a primary mouse anti-human E-cad monoclonal antibody (Abcam, Hong Kong, China), a primary
Techniques: Immunohistochemistry
Journal: Gastroenterology Research and Practice
Article Title: Peritoneal Metastatic Cancer Stem Cells of Gastric Cancer with Partial Mesenchymal-Epithelial Transition and Enhanced Invasiveness in an Intraperitoneal Transplantation Model
doi: 10.1155/2020/3256538
Figure Lengend Snippet: Immunocytofluorescence of E-cad, α -SMA, Survivin, Vimentin, MMP2, MMP9, GRHL2, ZEB1, and OVOL2 (×400) for the CSC-hGC, pMCSC-tGC [G1] , and pMCSC-tGC [G2] cells.
Article Snippet: The reagents used for immunohistochemical staining included a primary mouse anti-human E-cad monoclonal antibody (Abcam, Hong Kong, China), a primary
Techniques:
Journal: Gastroenterology Research and Practice
Article Title: Peritoneal Metastatic Cancer Stem Cells of Gastric Cancer with Partial Mesenchymal-Epithelial Transition and Enhanced Invasiveness in an Intraperitoneal Transplantation Model
doi: 10.1155/2020/3256538
Figure Lengend Snippet: Quantitative real-time PCR of E-cad, α -SMA, MMP9, Vimentin, GRHL2, OVOL2, ZEB1, and MMP2 for the CSC-hGC, pMCSC-tGC [G1] , and pMCSC-tGC [G2] cells.
Article Snippet: The reagents used for immunohistochemical staining included a primary mouse anti-human E-cad monoclonal antibody (Abcam, Hong Kong, China), a primary
Techniques: Real-time Polymerase Chain Reaction